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Aug 30, 2023

 

A polarizing microscope is a type of microscope used to study so-called transparent and opaque anisotropic materials. Any substance with birefringence can be clearly distinguished under a polarizing microscope. Of course, these substances can also be observed using staining, but some are impossible and must be observed using a polarizing microscope.


(1) Characteristics of polarizing microscope


(2) The basic principle of polarizing microscope

The principle of a polarizing microscope is relatively complex, and I will not introduce it too much here. A polarizing microscope must have the following accessories: a polarizing mirror, a polarizing mirror, a compensator or phase plate, a dedicated stress-free objective, and a rotating stage.


(3) The method of polarizing microscopy

a. Orthoscope, also known as distortionless microscopy, is characterized by the use of low-power objective lenses without the use of Bertrand Lenses. The subject can be directly studied using polarized light. At the same time, to reduce the illumination aperture, push open the upper lens of the condenser. Normal phase microscopy is used to check the birefringence of objects.



(4) Requirements for polarizing microscope devices




c. Focusing lens: In order to achieve parallel polarization, a swing-out condenser that can push out the upper lens should be used.



(5) Requirements for polarizing microscopy



 

2 Electronic Microscope

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